BioSciences - Polysciences, Inc.

Flow Cytometry
Bangs Flow Cytometry Standards
Microspheres & Particles
In order to perform reliable flow cytometric analyses, the instrument must be properly set up and
its performance validated. Bangs Flow Cytometry Standards are designed to help achieve these
goals and are presented in the following categories: Instrument Set-Up, Calibration, Validation and
Fluorescence Quantitation. Most of the Bangs Flow Cytometry Standards are available in three
convenient sizes: Trial (A Size: 20 tests, 1ml), Standard (B Size: 100 tests, 5ml) and Economy (C
Size: 280 tests, 14ml). A test requires one drop (50µl) of particle suspension, which is equivalent
to ~100,000 particles. Bangs Flow Cytometry Standards are ~7-9µm in diameter (unless otherwise
noted) to approximate the size of human lymphocytes. QuantumPlex kits for particle-based suspension
assays are also featured in this section.
Instrument Set-up
General Fluorescence Reference Standards
Full Spectrum Multi-color Reference Standards are highly uniform microspheres which fluoresce over a broad range
of the spectrum to provide several reference positions in multi-detector analysis. Used to properly
align flow cytometers excited from UV to near IR, this standard emits over the entire spectrum.
Catalog # Size
Tests
20 BLI885A-1 1 ml
100 BLI885B-5 5 ml
280 BLI885C-14 14 ml
Ultra Rainbow Fluorescent Particles
Internally labeled with a mixture of fluorophores which enables them to be excited at wavelengths
from 365nm to 650nm.
Tests Nom. Dia.
Ultra Rainbow Fluorescent Particles 20 ~3.8µm BLI610A-1 1 ml
100 BLI610B-5 5 ml
280 BLI610C-14 14 ml
Ultra Rainbow Fluorescent Particles 20 ~10.2µm BLI611A-1 1 ml
100 BLI611B-5 5 ml
280 BLI611C-14 14 ml
Time Delay Calibration Standard .............................................
Intended for use in assessing the delay between blue and red lasers. It features ~6µm microspheres
dyed with a fluorophore that is excited with 488nm or 635nm excitation and exhibits red/far-red
emission.
BLI830A-1 1 ml
BLI830B-5 5 ml
BLI830C-14 14 ml
Fluorescence Reference Standards
Fluorescence Reference Standards are labeled with specific fluorochromes so that they give rise to
the same fluorescence spectra as cells labeled with the same fluorochromes. Fluorescence intensity
is similar to biological samples.
Tests
Certified Blank (no label) Reference Standard 20 BLI890A-1 1 ml
100 BLI890B-5 5 ml
280 BLI890C-14 14 ml
Autofluor Reference Standard 20 BLI910A-1 1 ml
100 BLI910B-5 5 ml
280 BLI910C-14 14 ml
Acridine Orange Reference Standard 20 BLI897A-1 1 ml
100 BLI897B-5 5 ml
280 BLI897C-14 14 ml
For more information please call (800) 523-2575 or visit: polysciences.com 271