BioSciences - Polysciences, Inc.

Protein Conjugated Microspheres
Microspheres & Particles
Streptavidin Conjugated Microspheres
Streptavidin is covalently coupled to undyed and Fluoresbrite ® YG (excitation: 441nm, emission:
486nm) polystyrene microspheres. Packaged as 1.25% solids (w/v) aqueous suspensions.
Technical Data Sheet #616
Catalog # Size
Streptavidin Conjugated Microspheres A2dmw
Requires Cold Pack.
Streptavidin Microspheres
Nom. Dia.
1.0µm 24162-1 1 ml
24162-5 5 ml
Streptavidin Microspheres 2.0µm 24160-1 1 ml
24160-5 5 ml
Streptavidin Microspheres 6.0µm 24158-1 1 ml
24158-5 5 ml
Streptavidin Fluoresbrite ® YG Microspheres 1.0µm 24161-1 1 ml
24161-5 5 ml
Streptavidin Fluoresbrite ® YG Microspheres 2.0µm 24159-1 1 ml
24159-5 5 ml
Streptavidin Fluoresbrite ® YG Microspheres 6.0µm 24157-1 1 ml
24157-5 5 ml
Protein Coupling Kits for Microspheres
We offer kits for covalently coupling antibodies and other proteins to Polybead ® microspheres
greater than 0.5µm in diameter. One kit contains buffers and other reagents for coupling proteins
to carboxylate microspheres, while the other kit contains buffers and reagents for coupling proteins
to amino and blue dyed microspheres. The contents of each kit are sufficient for coupling proteins
to at least fifty 0.5ml samples (2.5% solids [w/v] in aqueous suspension) of microspheres. A detailed
instruction sheet is enclosed with each kit. Microspheres must be purchased separately.
Glutaraldehyde Kit for Amino Beads & Blue Dyed Beads BIX7dw . . . . . . . . . . . . . . . . . .
Kit contains 3 x 225ml Phosphate Buffered Saline, 2 x 10ml ampoules 25% EM Grade Glutaraldehyde,
60ml 0.2M Ethanolamine in Phosphate Buffered Saline, 60ml Bovine Serum Albumin solution,
60ml Storage Buffer and a labeled mixing and storage bottle. No beads are included in this kit.
Technical Data Sheets #238G and #410
Glutaraldehyde Kit with Hollow Fiber Filtering System BIX7dw ...................
Kit includes a hollow fiber separation device to aid in the washing and isolation steps. Microspheres
of 0.1µm-0.5µm diameter can be handled easily without laborious high speed centrifugation
techniques. Device may not be suitable for microspheres smaller than 0.1µm. For microspheres
smaller than 0.1µm, use Cat. #19540 along with dialysis tubing. No beads are included in this kit.
Technical Data Sheets #238G and #606
PolyLink Kit with Hollow Fiber Filtering System BVW7dw ........................
Kit includes a hollow fiber separation device to aid in the washing and isolation
steps. Microspheres of 0.1µm-0.5µm diameter can be handled easily without
laborious high speed centrifugation techniques. For microspheres less than
0.1µm, use Cat. #24350 with dialysis tubing. No beads are included in this kit.
Technical Data Sheets #606 and #853
PolyLink Protein Coupling Kit HO6bf ..........................................
Kit enables researchers to quickly couple proteins to carboxylated microspheres
in two hours or less. The procedure provided with the kit has been optimized
for polymer microspheres 1µm or larger. Kit contains 55ml Coupling Buffer,
45ml Wash/Storage Buffer and 0.75g EDAC. Each kit is sufficient for 50 coupling
reactions of 200-500µg of protein per reaction. No beads are included in
this kit. Technical Data Sheet #644
19540-1 1 kit
23964-1 1 kit
24818-1 1 kit
24350-1 1 kit
For more information please call (800) 523-2575 or visit: polysciences.com 267