BioSciences - Polysciences, Inc.

Histology & Microscopy
BioSciences
De-Calcify Block Solution BH4g ..............................................
Original decalcification thoroughness and condition of the specimen is dependent
on the original decalcification solution. Use De-Calcify Block Solution
as a fast microtome soak that saves you time and money by allowing you to
salvage problem blocks right at your cutting station.
Benefits:
Enhances ribbon cutting, useful for H&E and routine staining, fast soaking procedure,
ready-to-use formulation. Environmentally safe and biodegradable.
Poly-NoCal B4g ............................................................
Poly-NoCal is a specially formulated solution of high purity hydrochloric acid and EDTA. Designed
for rapid decalcification of fixed tissue sections, our formulation also includes buffers to help
prevent cellular swelling or distortion. Technical Data Sheet #523
Poly-NoCal & Fixative BHM6g ................................................
Poly-NoCal & Fixative is a formic acid and formaldehyde based solution designed to fix and decalcify
in one easy, convenient step. Small histological specimens, such as bone marrow, will achieve softening
sufficient for sectioning in four hours. Technical Data Sheet #523
Poly-NoCal End Point Determination Kit .......................................
Control of decalcification is an important part of tissue morphology. Over-decalcification
interferes with nuclear and cytoplasmic staining and also destroys cell
structure. Under-decalcification causes poor dehydration and infiltration during
processing and also results in sectioning problems. The Poly-NoCal End Point
Determination Kit includes all the necessary materials you need to determine
endpoint decalcification , accurately, consistently and conveniently. While actual
use conditions may vary, each kit typically performs approximately 200 tests,
helps to save you time and money and increases productivity.
Kit Contains: 500ml 5% Ammonium hydroxide, 250ml 5% Ammonium oxalate, pH papers and
measuring vials (components are also sold separately). Technical Data Sheet #524
Super Decalcifier I: Delicate BH4g .............................................
Gold Standard Series: Super Decalcifier I: Delicate is recommended for use
with delicate specimens and specimens (such as bone marrow biopsies (core)
that are to be submitted to IHC, cytochemistry and special stain procedures.
Biodegradable.
Benefits:
• Save Time - Decalcify in as little as 3 hours!
• Ready-to-use decalcifier for human and animal tissues
• Recommended for IHC, special stains and routine stains
Super Decalcifier II: Heavy Duty BH4g .........................................
For hard compact bone, (i.e. femur heads, above- the-knee amputation (AKA)
and below-the-knee amputation (BKA). Super Decalcifier II: Heavy Duty is an
extremely effective and versatile decalcifier that can be used in specific lab routines.
As with most acids, nucleic acids in the cell can become subject to ribonuclease
digestion, resulting in a loss of basophilic properties. Careful monitoring
should be used with Super Decalcifier II: Heavy Duty to avoid over decalcifica-
tion. Most decalcification occurs in approximately 4-6 hours or less, depending
on the thickness and density of the specimens. Overnight decalcification should
be avoided. Super Decalcifier II: Heavy Duty is not recommended for IHC.
Biodegradable.
Benefits:
• Save Time - Decalcify in as little as 3 hours!
• Ready-to-use decalcifier for human and animal tissues
Human Degenerative Joint
Disease, decalcified with Super
Decalcifier I Delicate, stained
with H & E, 10X
Human Degenerative Joint
Disease, decalcified with Super
Decalcifier II - Heavy Duty,
stained with H & E, 40X
Catalog # Size
24903-250 250 ml
24903-500 500 ml
24164-1 1 liter
24163-1 1 liter
24119-1 1 kit
24888-500 500 ml
24888-1 1 liter
24888-6 6x1 liter
24887-500 500 ml
24887-1 1 liter
24887-6 6x1 liter
For more information please call (800) 523-2575 or visit: polysciences.com 17