BioSciences - Polysciences, Inc.

Histology & Microscopy
BioSciences
Sirius red, C.I. 35780 [2610-10-8] H4g ..........................................
(Direct red 80) MW 1373.09
Cardiac muscle stain. Stain Technol., 62, 23 (1987); J. Histochem. Cytochem., 41, 465 (1993)
Sudan Black B, C.I. 26150, Certified [4197-25-5] .................................
MW 456.54 C 29 H 24 N 6
Sudan Black B is useful for staining neutral triglycerides and lipids on frozen sections and some lipoproteins
on paraffin sections. It has the appearance of a dark brown to black powder with maximum
absorption at 596-605 nm and melting point 120-124 °C. It stains blue-black. Sudan Black B is one
of the dyes used for Sudan staining. Similar dyes include Oil Red O, Sudan III, and Sudan IV.
J. Biochem. Biophys. Methods 21, 9, (1990) Abstract, Penny, DP et al, Analysis and testing of biological stains - The Biological
Stain Commission Procedures. Biotech. Histochem. 77(5&6), 237-275, (2002), Merck 13, 8970
Tetrachrome stain, certified (MacNeal) H4g ....................................
Blood stain similar to Wright’s stain. Also useful for staining bone sections.
Conn’s Biological Stains, Williams & Wilkins, 9th ed., 1977, p 606
Thionin, C.I. 52000, certified [78338-22-4] U3g ..................................
MW 287.34
A metachromatic, cationic thiazine dye that is used widely as a biological stain, especially in histology.
Useful as a metachromatic stain for brain tissue and as a Nissl stain. Stain Technol., 25, 195 (1950); 49, 49 (1974)
Toluidine blue O, C.I. 52040, certified [92-31-9] U5g .............................
MW 305.83 l max 626nm
A metachromatic, cationic thiazine dye that is widely used in in vitro biological applications. Also
used in techniques for DNAase detection.
Stain Technol., 18, 35 (1943); 38, 281 (1963); J. Clin. Microbiol., 21, 195 (1985); Arch. Surg., 95, 16 (1967)
Toluidine blue O, C.I. 52040, purified [92-31-9] U5g .............................
MW 305.83 l max 626nm
Useful for staining RNA, oligodeoxynucleotides, proteins and glycosaminoglycans.
Nature, 213, 1133 (1967); Anal. Biochem., 46, 156 (1972)
Trypan blue, C.I. 23850 [72-57-1] HVWX6g ......................................
(Direct blue 14; Niagara blue 3B) MW 960.83 mp >300° l max 607nm
Trypan blue has been widely used to assess the viability of eukaryotic cells. This method is often
called trypan blue exclusion. Nonviable cells will exhibit a marked concentration of this blue dye in
their nuclei. Tissue culture labs will find it extremely useful for determining the health and density
of cell lines. Other studies have demonstrated trypan blue as an inactivating agent for measles and
herpes simplex viruses and as a teratogenic agent in rats.
J. Histochem. Cytochem., 18, 581 (1970); J. Gen. Virol., 64, 1365 (1983); Teratology, 26, 289 (1982)
Wright stain, certified [68988-92-1] U4g .......................................
Useful for staining blood films and malarial parasites in blood films.
Clearing & Dehydration Reagents
Acetone
[67-64-1] CH6g CH COCH 3 3
For hardening and dehydrating tissues. It can also be used for the extraction of various principles
from animal and plant substances. Merck Index 11, 58
Catalog # Size
99.5% - EM grade and Histology grade, 99.5% minimum 01921-1 1 liter
01921-6 6 pints
01921-4 4 liters
Glass Distilled, 99.7% minimum 08523-1 1 liter
08523-4 4 liters
Alcohol Reagent, 100% - Histology Grade C5g .................................
Composed of 90 parts ethyl alcohol, 5 parts methyl alcohol and 5 parts isopropyl alcohol, v/v blend.
Suitable for most histological procedures.
09400-10 10 g
09400-25 25 g
25008-25 25 g
02783-5 5 g
01220-10 10 g
01234-25 25 g
15931-10 10 g
01294-100 100 g
02785-25 25 g
02785-100 100 g
09860-1 1 gal
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