BioSciences - Polysciences, Inc.
BioSciences - Polysciences, Inc.
BioSciences - Polysciences, Inc.
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14<br />
Histology & Microscopy<br />
<strong>BioSciences</strong><br />
Methylene blue chloride, C.I. 52015, certified [61-73-4] H5g ......................<br />
MW 373.9<br />
Sensitive stains for RNA, very good nuclear stain, used in many in vitro diagnostic applications.<br />
Technical Data Sheet #727 Clark, G., Staining Procedures, 4th ed., Williams and Wilkins: Baltimore, 1981<br />
Neutral red, C.I. 50040, certified [553-24-2] U5g ................................<br />
(Basic red 5; 3-Amino-7-dimethylamino-2-methylphenazine hydrochloride; Toluidine red)<br />
MW 288.78 l max 540nm<br />
Used as an indicator. pH range 6.8 (red)-8.0 (yellow). Also used as a biological stain. Merck Index 11, 6395<br />
Nuclear fast red, C.I. 60760 [6409-77-4] HU5g ...................................<br />
MW 357.28 l max 518nm<br />
Nuclear fast red staining is a simple method of nuclear chromatin staining and is mainly used for<br />
high-contrast counterstaining for histological applications. Clin. Chim. Acta, 2, 567 (1957)<br />
Oil Red O, C.I. 26125, certified [1320-06-5] A2g .................................<br />
MW 408.5 mp 120°<br />
A post-electrophoresis stain for lipoproteins on cellulose acetate plates. Z. Clin. Chem. Clin. Biochem., 7, 540 (1969)<br />
Orange G, C.I. 16230, certified [1936-15-8] U4g .................................<br />
MW 452.38<br />
Mallory’s stain for collagen in connective tissue. Also useful as a stain for granules, elastic fibers,<br />
mast cells, and pollen tubes. General histology and cytology counterstain.<br />
Stain Technol., 13, 89 (1938); Am. J. Clin. Path., 20, 665 (1950)<br />
Pyronin Y, C.I. 45005, certified [92-32-0] U5g ...................................<br />
MW 302.8<br />
Used in combination with methyl green for the selective and differential staining of nucleic acids.<br />
The pyronin Y stains RNA red, while the methyl green stains DNA green. The combined methyl<br />
green-pyronin Y stain is a useful histochemical reagent. Pyronin Y can also be used as a tracking dye<br />
for polyacrylamide gel electrophoresis.<br />
Stain Technol., 27, 233 (1952); Clark, G., Staining Procedures, 4th ed., Williams and Wilkins 1981; p199<br />
Rhodamine 6G, C.I. 45160 [989-38-8] ..........................................<br />
(Basic Red 1) MW 479.01 C 28 H 31 N 2 O 3 CI<br />
Used as a tracer dye within water to determine the rate and direction of flow and transport.<br />
Rhodamine dyes fluoresce and thus can be detected easily, used extensively in fluorescence microscopy,<br />
flow cytometry, fluorescence correlation spectroscopy and ELISA.<br />
Rose Bengal, C.I. 45440, Certified [632-69-9] ....................................<br />
MW 1017.64 C 20 H 2 CI 4 I 4 Na 2 O 5<br />
Rose Bengal can be used as an alternative to phloxine B in Kreyberg’s stain for keratin and mucus.<br />
Useful for detecting bacteria in soil. Merck 13, 8343, Beilstein 19, IV, 2926<br />
Rosolic Acid, C.I. 43800 [603-45-2] .............................................<br />
MW 290.31 C 19 H 14 O 3 l max 482nm<br />
Rosolic acid is an organic compound, forming yellowish or deep-red crystals with greenish metallic<br />
luster. Practically insoluble in water and freely soluble in alcohol. Soluble in strong acids to form a<br />
yellow solution or in aqueous alkalis to form carmine red solutions. Due to this behavior, it is suitable<br />
to be used as a pH indicator with pH transition range 5.0 - 6.8. Merck 13, 885, Beilstein 8, IV, 2646<br />
Safranin O, C.I. 50240, certified [477-73-6] H5g .................................<br />
MW 350.85<br />
A general biological stain. Used as a nuclear stain for histological studies. Also<br />
used as a cationic lipophilic probe and in the detection of glycosaminoglycans<br />
and proteoglycans.<br />
Eur. J. Biochem., 194, 389 (1990); Biochem. Int., 24, 485 (1991)<br />
For more information please call (800) 523-2575 or visit: polysciences.com<br />
Francisella tularensis using a Safranin stain, Magnified 1000X.<br />
Image courtesy of CDC Public Health Library / Dr. P. B. Smith.<br />
Catalog # Size<br />
04220-50 50 g<br />
00915-25 25 g<br />
09773-5 5 g<br />
06317-25 25 g<br />
06317-100 100 g<br />
00968-100 100 g<br />
18614-5 5 g<br />
18614-25 25 g<br />
25004-25 25 g<br />
25004-50 50 g<br />
25005-25 25 g<br />
25006-25 25 g<br />
02782-25 25 g
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