BioSciences - Polysciences, Inc.
BioSciences - Polysciences, Inc.
BioSciences - Polysciences, Inc.
Create successful ePaper yourself
Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.
Histology & Microscopy<br />
<strong>BioSciences</strong><br />
Fluorescein isothiocyanate, Certified, FITC [3326-32-7] HPRS5e ....................<br />
(Fluorescein 5-isothiocyanate; FITC) MW 389.4 mp >360°<br />
Moisture sensitive isomer I, 90% minimum, laser grade. Reagent widely used<br />
for fluorescent labeling of proteins. Also used in the fluorescent antibody<br />
technique for identification of pathogens. Used in Fluorescent In-situ Hybridization<br />
(FISH) procedures.<br />
Am. J. Pathol., 34, 1081 (1958); Methods in Enzymology, 26, 28 (1972); Anal. Biochem., 57, 227 (1974)<br />
Rat dorsal ganglion, triple-labeled with FITC (CGRP) secondary antibodies, Texas Red ®<br />
(extracellular matrix) and avidin-Pacific Blue (biotinylated isolectin GS-I-B4). Photo: Jeffrey C.<br />
Petruska, Dept. of Neuroscience, and Richard D. Johnson, PhD, Dept. of of Physiological<br />
Science and Neuroscience, University of Florida Brain Institute.<br />
Fuchsin, basic, certified, C.I. 42500 [569-61-9] HM4g .............................<br />
MW 323.83 l max 542-548nm<br />
For staining bacilli in tissue. Coupling agent for ultrastructural localization of esterases.<br />
Technical Data Sheet #727 Stain Technol., 19, 45 (1944); J. Cell Biol., 29, 361 (1966)<br />
Fuchsin, basic, certified, C.I. 42510 [632-99-5] HM4g .............................<br />
MW 337.86<br />
For staining glycoproteins and glycosaminoglycans in electrophoresis gels. More permanent bands<br />
are produced by post-staining with naphthol blue black. Tracking dye for proteins in acidic gel<br />
systems. Certified for use in staining bacterial flagella. Anal. Biochem., 51, 152; 56, 361 (1973)<br />
Gallamine blue, C.I. 51045 [1563-02-6] U5g .....................................<br />
(Mordant blue 45) MW 335.73<br />
Used as a nuclear stain. Also used for detecting calcium in tissues.<br />
Stain Technol., 3, 28 (1928); J.R. Microsc. Soc., 69, 20 (1944)<br />
Gentian Violet, C.I. 42555, USP ...............................................<br />
MW 407.98 C 25 H 30 N 3 Cl<br />
Gentian Violet is the primary agent used in staining bacteria by the Gram Stain method.<br />
Giemsa Stain Powder, Certified [51811-82-6] ...................................<br />
MW 291.80 C 14 H 14 CIN 3 S<br />
Giemsa stain is a classical blood film stain for peripheral blood smears and bone marrow specimens,<br />
used to visualize chromosomes, stains fungus histoplasma and identifies mast cells.<br />
Hematein, C.I. 75290 (Hydroxybrasilein) [475-25-2] U4g .........................<br />
MW 300.26 mp 200°<br />
An indicator like hematoxylin; for staining animal tissue, particularly cell nuclei.<br />
Hematoxylin, C.I. 75290, certified (Natural black 1) [517-28-2] A2g ................<br />
MW 302.3<br />
Nuclear protein stain and glycogen stain. Also a general tissue stain for human, animal and VIR<br />
histology. Stain Technol., 14, 47 (1930); 62, 181 (1987)<br />
Light green SF yellowish, C.I. 42095, certified [5141-20-8] A5g ....................<br />
MW 792.85<br />
Used as a dye and as a biological stain. Certified for use as a counterstain in cytology. An important<br />
contrast stain for plasma as a critical component of Papanicolaou (PAP) stains. Stains collagen fibers<br />
when substituted for aniline blue in Masson’s trichrome.<br />
Merck Index, 11, 5361; Stain Technol., 6, 13 (1931); 14, 1 (1939); J. Technol. Methods, 25, 1 (1945), Churchill Livingstone,<br />
Theory and Practice of Histo. Tech., Bancroft & Stevens, p 466<br />
Malachite Green Oxalate, C.I. 42000, Certified [2437-29-8] .......................<br />
MW 463.50 C 23 H 25 N 2 • C 2 HO 4 •1/2 C 2 H 2 O 4<br />
Malachite Green Oxalate is used as a counterstain for paraffin embedded botanical material and staining<br />
bacterial polysaccharides and spores. Also used as a contrast stain in the Ziehl-Neelsen procedure.<br />
Penny, DP et al, Analysis and testing of biological stains - The Biological Stain Commission Procedures. Biotech. Histochem.<br />
77(5&6), 237-275, (2002)<br />
Methyl green, C.I. 42590, certified [7114-03-6] U5g ..............................<br />
MW 472.5 mp 233° (dec.)<br />
Used with Pyronin Y to stain nuclei and nucleoli differentially.<br />
Stain Technol, 26, 205 (1951); Mori & Lennert, Electron Microscopic Atlas of Lymph Node Cytology, Springer, 1969<br />
Catalog # Size<br />
00373-1 1 g<br />
06342-25 25 g<br />
00635-25 25 g<br />
05587-1 1 g<br />
24999-25 25 g<br />
24998-25 25 g<br />
01005-25 25 g<br />
02749-25 25 g<br />
02749-100 100 g<br />
02753-25 25 g<br />
25002-25 25 g<br />
02760-25 25 g<br />
For more information please call (800) 523-2575 or visit: polysciences.com 13
Change language