BioSciences - Polysciences, Inc.
BioSciences - Polysciences, Inc.
BioSciences - Polysciences, Inc.
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10<br />
Buffers<br />
<strong>BioSciences</strong><br />
1M Sodium Bicarbonate-Sodium Carbonate Buffer, pH 9.6±0.2, 10X Concentrate A2d<br />
Dilute 1–>10 with deionized water to get buffer at working concentration.<br />
Suitable for use in protocols for covalent coupling of proteins to carboxylate<br />
microspheres.<br />
Sodium Phosphate Storage Buffer A2d ........................................<br />
0.02M PBS with biocide (0.1% sodium azide), blocking protein BSA, and glycerol. For the storage<br />
of protein bound microspheres.<br />
StainRITE May-Grünwald Giemsa Phosphate Buffer pH 7.2 .....................<br />
For use as a buffer in May-Grünwald, Wright Stain, Wright-Giemsa, Giemsa and Leishman staining<br />
procedures.<br />
StainRITE Wright-Giemsa Stain Phosphate Buffer pH 6.8 .......................<br />
Yields satisfactory staining results every time when used with Wright-Giemsa stains. The buffer used<br />
in the Wright-Giemsa staining protocol needs to be pH 6.8 or results will be unsatisfactory. If the<br />
buffer is too acidic the stain will be too red and nuclei will be too light; if it is too basic the stain will<br />
be too blue and cytoplasmic detail will be indistinct.<br />
Technical Data Sheet #813 & #815<br />
StainRITE Wright Stain Phosphate Buffer pH 6.8 ...............................<br />
Yields satisfactory staining results every time when used with the Wright staining method. The<br />
buffer used in the Wright staining protocol needs to be pH 6.8 or results will be unsatisfactory.<br />
If the buffer is too acidic the stain will be too red and nuclei will be too light; if it is too basic the<br />
stain will be too blue and cytoplasmic detail will be indistinct.<br />
Tris base, Chemzymes Ultra Pure [77-86-1] H5g . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .<br />
(Tris[hydroxymethyl]aminomethane ) MW 121.14 mp 169-173°<br />
Ultra Pure Tris base, recrystallized is the buffer of choice for a variety of biochemical applications. Tris<br />
displays optimum performance in the pH range of 7-9, the range in which many enzymes exhibit<br />
maximum activity. It is used in enzyme purification and assay protocols. It is also widely used as a<br />
buffer for enzyme digestion of DNA, and is ideally suited for use as an electrophoresis buffer for<br />
bothe agarose and polyacrylamide gelso used to analyze DNA fragments, RNA, and proteins.<br />
Purity: 99.9%. Technical Data Sheet #502 Anal. Chem., 37, 1291 (1965)<br />
Tris Buffered Saline (TBS), pH 8.0±0.2, 10X Concentrate A2d .....................<br />
Dilute 1–>10 with deionized water to get buffer at working concentration.<br />
Tris Buffered Saline (TBS), pH 8.0±0.2, 1X Powdered Blend H4g ..................<br />
Dissolve the contents of each pouch in a liter of deionized water to get 0.05M<br />
Tris-HCl, 0.15M Sodium chloride.<br />
Tris·HCl, Chemzymes Ultra Pure [1185-53-1] HK3g ..............................<br />
(Tris[hydroxymethyl]aminomethane hydrochloride ) MW 157.64<br />
An alternative to Tris base in the preparation of Tris buffers. Purity: 99% Technical Data Sheet #503<br />
For more information please call (800) 523-2575 or visit: polysciences.com<br />
Catalog # Size<br />
24095-500 500 ml<br />
24095-1 1 liter<br />
24095-4 4x1 liter<br />
24171-20 20 ml<br />
25032-1 1 liter<br />
25032-4 4 liters<br />
24984-1 1 liter<br />
24984-4 4 liters<br />
24989-1 1 liter<br />
24989-4 4 liters<br />
23483-100 100 g<br />
23483-250 250 g<br />
23483-500 500 g<br />
24082-500 500 ml<br />
24082-1 1 liter<br />
24083-5 5 pouches<br />
24083-10 10 pouches<br />
23484-100 100 g<br />
23484-250 250 g<br />
23484-500 500 g
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