BioSciences - Polysciences, Inc.
BioSciences - Polysciences, Inc.
BioSciences - Polysciences, Inc.
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Buffers<br />
<strong>BioSciences</strong><br />
Tris-Borate-EDTA Buffer (TBE Buffer) pH 8.3±0.2, 1X Powdered Blend H4g .........<br />
Dissolve the contents of each pouch in a liter of deionized water to get 0.089M<br />
Tris/Borate, 0.002M Disodium EDTA.<br />
Tris-Borate-EDTA Buffer (TBE Buffer), pH 8.3±0.2, 10X Concentrate A2d ...........<br />
Dilute 1–>10 with deionized water to get buffer at working concentration. Suitable for use in gel<br />
electrophoresis for nucleic acid analysis.<br />
J. Sambrook et al., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory (1989), p. 6.7, B.23.<br />
Tris-Glycine Buffer (TG Buffer), pH 8.3±0.2, 10X Concentrate A2d .................<br />
Suitable for use in Polyacrylamide gel electrophoresis and Western blotting. Dilute 1–>10 with<br />
deionized water to get buffer at working concentration. H.Towbin et al. Proc. Nat. Acad. Sci. USA, 75: 4350 (1979<br />
Tris-Glycine Buffer (TG Buffer), pH 8.3±0.2, 10X Concentrate A2d .................<br />
Dissolve the contents of each pouch in a liter of deionized water to get 0.025M Tris base, 0.192M Glycine.<br />
1M Glycine-HCl Buffer, pH 2.7±0.1, 10X Concentrate A2d ........................<br />
Dilute 1–>10 with deionized water to get buffer at working concentration.<br />
Michel’s Buffer A2mw .......................................................<br />
Holding medium for tissue undergoing immunofluorescent studies.<br />
Not to be used for tissues used in Fluorescent In situ.<br />
Phosphate Buffered Saline (PBS), pH 7.4±0.2, 1X Powdered Blend H4g ............<br />
Dissolve the contents of each pouch in a liter of deionized water to get 0.01M<br />
Sodium phosphate, 0.15M Sodium chloride.<br />
0.05M Phosphate-Citrate Buffer, pH 5.0±0.2, 1X Powdered Blend H4g .............<br />
Dissolve the contents of each pouch in a liter of deionized water.<br />
PolyTransport Buffer A2g ...................................................<br />
Used to transport and preserve specimens from surgery to the point of grossing<br />
and fixation prior to further processing. PolyTransport Buffer prevents proteins<br />
from denaturing or crosslinking and prevents bacterial growth. PolyTransport Buffer<br />
also contains a cryoprotectant to prevent ice crystal formation during rapid<br />
freezing techniques and a membrane stabilizer to prevent membrane lysis. No<br />
washing of specimens necessary prior to placing in fixative or rapid freezing.<br />
Technical Data Sheet #631<br />
Tris-Glycine-SDS Buffer (TGS Buffer), pH 8.3±0.2, 10X Concentrate A2d ............<br />
Dilute 1–>10 with deionized water to get buffer at working concentration. Suitable for use in<br />
SDS-Polyacrylamide gel electrophoresis. U.K. Laemmli., Nature, 227: 680 (1970).<br />
0.1M Sodium Bicarbonate-Sodium Carbonate Buffer, pH 9.6±0.2, 1X Powdered Blend H4g<br />
Dissolve the contents of each pouch in a liter of deionized water.<br />
Catalog # Size<br />
24087-5 5 pouches<br />
24087-10 10 pouches<br />
24086-500 500 ml<br />
24086-1 1 liter<br />
24088-500 500 ml<br />
24088-1 1 liter<br />
24089-5 500 ml<br />
24089-10 1 liter<br />
24074-500 500 ml<br />
24074-1 1 liter<br />
24353-500 500 ml<br />
24353-1 1 gal<br />
24081-5 5 pouches<br />
24081-10 10 pouches<br />
24079-5 5 pouches<br />
24079-10 10 pouches<br />
24311-250 250 ml<br />
24311-500 500 ml<br />
24090-500 500 ml<br />
24090-1 1 liter<br />
24094-5 5 pouches<br />
24094-10 10 pouches<br />
For more information please call (800) 523-2575 or visit: polysciences.com 9
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