BioSciences - Polysciences, Inc.

8
Buffers
BioSciences
0.5M Acetate Buffer, pH 4.6±0.2, 10X Concentrate A2d ..........................
Dilute 1–>10 with deionized water to get buffer at working concentration.
0.5M Borate Buffer, pH 8.5±0.2, 5X Concentrate A2d ............................
Dilute 1–>5 with deionized water to get buffer at working concentration.
Suitable for use in protocols for coupling proteins to polystyrene microspheres.
Cacodylic acid, free acid [75-60-5] KP7bg .......................................
(Dimethylarsinic acid) MW 138.0 mp 198-200° Requires Poison Pack.
Cacodylic acid, sodium salt trihydrate [6131-99-3] MV7g .........................
(Sodium cacodylate) MW 214.03
Dissolve in distilled or deionized water heated to 60°C, under a hood.
Requires Poison Pack.
Cacodylic acid, sodium salt, solution, 0.2M, pH 7.4 H4d .........................
Buffer for light and electron microscopy fixatives; e.g., osmium tetroxide,
formaldehyde, glutaraldehyde, Karnovsky’s fixative.
0.5M Citrate-Citric Acid Buffer, pH 4.6±0.2, 10X Concentrate A2d .................
Dilute 1–>10 with deionized water to get buffer at working concentration.
0.05M Citrate-Citric Acid Buffer, pH 4.6±0.2, 1X Powdered Blend H4g .............
Dissolve the contents of each pouch in a liter of deionized water.
Tris-Acetate-EDTA Buffer (TAE Buffer), pH 8.3±0.2, 10X Concentrate A2d ..........
Dilute 1–>10 with deionized water to get buffer at working concentration. Suitable for use in gel
electrophoresis for nucleic acid analysis.
1M Tris Base A2d
(Tris[hydroxymethyl]aminomethane) MW 121.14 mp 169-173°
Ultra Pure Tris base, recrystallized is the buffer of choice for a variety of biochemical applications. Tris
displays optimum performance in the pH range of 7-9, the range in which many enzymes exhibit
maximum activity. It is used in enzyme purification and assay protocols. It is also widely used as
a buffer for enzyme digestion of DNA, and is ideally suited for use as an electrophoresis buffer for
both agarose and polyacrylamide gels used to analyze DNA fragments, RNA and proteins.
For more information please call (800) 523-2575 or visit: polysciences.com
Catalog # Size
1M Tris Base, pH 10.7±0.2 24098-500 500 ml
24098-1 1 liter
1M Tris Hydrochloride, pH 4.1±0.2 24099-500 500 ml
24099-1 1 liter
1mM EDTA Buffer for Antigen Retrieval, pH 8.0 A2d & A3d ......................
1mM EDTA buffer for use on formalin-fixed, paraffin-embedded tissue sections for target retrieval
prior to immunohistochemistry (IHC) procedure. Technical Data Sheet #25080A and #25080B
24075-1 1 liter
24075-500 500 ml
24092-500 500 ml
24092-1 1 liter
03880-100 100 g
01131-25 25 g
01131-100 100 g
01131-500 500 g
18661-100 100 ml
18661-500 500 ml
24076-500 500 ml
24076-1 1 liter
24077-5 5 pouches
24077-10 10 pouches
24084-1 1 liter
24084-4 4x1 liter
25080-1 1 kit