BioSciences - Polysciences, Inc.
BioSciences - Polysciences, Inc.
BioSciences - Polysciences, Inc.
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Molecular Biology<br />
<strong>BioSciences</strong><br />
Filipin (from Streptomyces filipinensis) [11078-21-0] HO6d .......................<br />
Polyene antibiotic fluorochrome for cholesterol determination. Used as an<br />
antifungal agent. Cytometry, 8, 146 (1987)<br />
Fluorescein diacetate [596-09-8] A2f ..........................................<br />
(Diacetylfluorescein; 3,6-Diacetoxyfluoran) MW 416.39 mp 198-201° l max 498nm<br />
Lipase substrate. Also a fluorescent substrate in studies on membrane permeability and intracellular<br />
interactions. Anal. Chem., 36, 409 (1964); J. Biol. Chem., 258, 6380 (1983)<br />
Hydroethidine (Dihydroethidium bromide) [104821-25-2] HU5cd .................<br />
MW 315.5 mp 202-206 ° Exc. max: 365nm Emiss. max: 420nm<br />
Reduced ethidium bromide. A vital stain. Enters and stains living cells without<br />
cellular trauma. Double staining system. Stains cytoplasm blue and chromatin<br />
red. Excellent cellular retention. Remains incorporated in chromatin<br />
with virtually no leakage. Essentially non-toxic. Shows no toxicity at levels<br />
useful for visualizing chromatin. Technical Data Sheet #351<br />
Biotechnology, 3, 4 (1985)<br />
Lissamine Rhodamine B sulfonyl chloride, ~99% [62796-29-6] H3g ................<br />
MW 577.1<br />
Fluorescent protein stain with bright orange fluorescence. Used to visualize actin filaments in living<br />
amoebas. Useful for waste water testing and various microbiological applications. J. Cell. Biol. 86, 590 (1980)<br />
Osmium ammine-B [48016-91-7] U5g ..........................................<br />
Stable DNA stain. J. Histochem. Cytochem., 37, 395 (1989)<br />
Propidium iodide [25535-16-4] HVWX6g .......................................<br />
MW 668.41 mp 220.225º<br />
Fluorescent marker. Used as a nuclear counterstain and for In situ Hybridization (ISH).<br />
J. Histochem. Cytochem., 35, 123 (1987)<br />
Rhodamine B isothiocyanate, mixture of isomers [36877-69-7] U5g ...............<br />
MW 536.1<br />
Fluorescent marker for proteins. Also used as a counterstain in conjunction with FITC.<br />
J. Bacteriol., 83, 1358 (1962); Arch. Biochem. Biophys., 133, 263 (1969)<br />
Toluidine blue O, C.I. 52040, certified [92-31-9] U5g .............................<br />
MW 305.83 l max 626nm<br />
A metachromatic, cationic thiazine dye that is widely used in in vitro biological applications. Also<br />
used in techniques for DNAase detection.<br />
Stain Technol., 18, 35 (1943); 38, 281 (1963); J. Clin. Microbiol., 21, 195 (1985); Arch. Surg., 95, 16 (1967)<br />
Toluidine blue O, C.I. 52040, purified [92-31-9] U5g .............................<br />
MW 305.83 l max 626nm<br />
Useful for staining RNA, oligodeoxynucleotides, proteins and glycosaminoglycans.<br />
Nature, 213, 1133 (1967); Anal. Biochem., 46, 156 (1972)<br />
XTT [111072-31-2] HU7ad ....................................................<br />
MW 673.5 (Tetraxolium XTT; Sodium 3’-[1-phenylamino-carbonyl]-<br />
3,4-tetrazolium]-bis[4-methoxy-6-nitro]benzenesulfonic acid hydrate)<br />
Useful in in vitro cell growth assays with possible applicability to a variety of<br />
problems in cellular pharmacology and biology. An improved colorimetric assay<br />
for cell proliferation and viability utilizing XTT has been reported. An XTT-based<br />
colorimetric cellular cytotoxicity assay for melanoma and other tumor cells has<br />
also been reported. Technical Data Sheet #486<br />
Cancer Res., 48, 4827 (1988); J. Immunol. Methods, 142, 257 (1991); 147, 153 (1992)<br />
Catalog # Size<br />
08707-50 50 mg<br />
08707-100 100 mg<br />
00615-5 5 g<br />
17084-50 50 mg<br />
08074-100 100 mg<br />
21033-100 100 mg<br />
03748-100 100 mg<br />
00374-250 250 mg<br />
01234-25 25 g<br />
15931-10 10 g<br />
19661-100 100 mg<br />
19661-500 500 mg<br />
For more information please call (800) 523-2575 or visit: polysciences.com 93
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