BioSciences - Polysciences, Inc.
BioSciences - Polysciences, Inc.
BioSciences - Polysciences, Inc.
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92<br />
Molecular Biology<br />
<strong>BioSciences</strong><br />
Cyanoditolyl Tetrazolium Chloride (CTC) [102568-47-8] HU6ad ....................<br />
MW 311.8 mp 230° ~100mg in 6.6cc water<br />
Exc max: 450nm Emiss max: 630nm<br />
Cyanoditolyl tetrazolium chloride has been used to measure the redox activity<br />
of tumor cells. It has also been employed for direct epifluorescent microscopic<br />
enumeration of respiring bacteria in food samples and environmental samples,<br />
especially water samples. Reveals a quantitative methodology for measuring<br />
marine bacteria. These methods yield 80% activity in 2 to 10 minutes. Cell<br />
samples mixed with CTC can be stored refrigerated or frozen in liquid nitrogen<br />
for at least 4 weeks without a significant loss of cells. Formazan fluoresces in<br />
solid state. Technical Data Sheet #486<br />
Histochem. J. 19, 21 (1987); Appl. Environ. Microbiol., 58, 1801 (1992); Appl. Environ. Microbiol., 65(5), 1966 (1999); Appl.<br />
Environ. Microbiol., 69(4), 2166 (2003); Flow Cytometric Analysis: Appl. Environ. Micro. June 1999, p. 2409-2417 Vol. 65 (6)<br />
5-Bromo-4-chloro-3-indolyl b-D-galactopyranoside [7240-90-6] U5f ...............<br />
MW 408.64 mp 230° (dec.)<br />
Histochemical substrate for b-galactosidase.<br />
J. Med. Chem., 7, 574 (1964); Histochemie, 37, 375, (1973); Hum. Gene Thor., 8, 1545 (1997)<br />
4’,6-Diamidino-2-phenylindole dihydrochloride (DAPI) [28718-90-3] U3acf ..........<br />
MW 350.25 l max 342nm<br />
A cationic fluorescent dye which specifically binds to adenine-thymine-rich<br />
DNA. Applications include detection of nanogram quantities of DNA in cellular<br />
homogenates, and cytofluorometric determination of the DNA base content in<br />
human chromosomes. Available in bulk quantities for OEM users at significant<br />
savings. Technical Data Sheet #444<br />
Nature, 253, 461 (1975); Anal. Biochem., 92, 497 (1979); Stain Technol.,<br />
60, 7 (1985); Eur. J. Biochem., 182, 437 (1989)<br />
DAB-4HCL (3-3’-Diaminobenzidine tetrahydrochloride) Immunochemical Grade [7411-49-6] HMVW7bg<br />
MW 360.1<br />
Chromogen of choice for immunoperoxidase techniques. The brown reaction<br />
product is highly insoluble, and will not diffuse from the site of localization. The<br />
color does not fade, and therefore, slides can be saved as a permanent record.<br />
The reaction product also chelates with osmium tetroxide and becomes electron<br />
dense for use in electron microscopy. By adding buffer directly to the DAB<br />
serum vial with a hypodermic syringe, contact with the solid DAB is avoided.<br />
Our DAB-4HCl is prepared to the highest purity standards. It is fully water soluble<br />
and may be used without further purification for immunocytochemistry and<br />
for endogenous oxidases and peroxidase. Technical Data Sheet #164<br />
Manual of Immunoperoxidase Techniques, 2nd Edition, ASCP Press, Chicago, 1987<br />
For more information please call (800) 523-2575 or visit: polysciences.com<br />
E. coli bacteria in CTC<br />
Mouse Kidney cells were stained with<br />
DAPI, Alexa Fluor ® 488 wheat germ<br />
agglutinin, Alexa Fluor ® 568phalloidin.<br />
Contributed by Walt Metcalfe, Molecular<br />
Probes, <strong>Inc</strong>.; photographed by Gregg<br />
Jarvis, Omega Optical, <strong>Inc</strong>.<br />
Human breast tissue stained<br />
with DAB in Peel-A-Way ®<br />
Micro-Cut Paraffin, 100X<br />
2’,7’-Dichlorofluorescein [76-54-0] H4g ........................................<br />
MW 401.21<br />
0.2% Solution in ethanol can be used for the detection of saturated and unsaturated lipids.<br />
Ethidium bromide [1239-45-8] H5g ............................................<br />
MW 394.32 mp 260-262° (dec.)<br />
(Homidium bromide; 3,8-Diamino-5-ethyl-6-phenylphenanthridium bromide; 2,7-diamino-<br />
10-ethyl-9-phenylphenanthridium bromide)<br />
Intercalates double-stranded nucleic acids; frameshift mutagen; fluorescent stain for nucleic acids in<br />
electrophoresis; used for DNA quantitation.<br />
J. Mol. Biol., 47, 419 (1970); Ann. Rev. Biochem., 44, 273 (1975); Dev. Biol., 108, 325 (1985)<br />
Fast Blue [74749-42-1] U7g ...................................................<br />
Fluorescent dye commonly used as a neuronal tracer and referred to as a<br />
hydrophilic retrograde tracer. Fast Blue can be used alone or with other<br />
types of fluorescent retrograde and anterograde tracer dyes. Other neuronal<br />
tracers such as True Blue, Evans Blue and Nuclear Yellow are commonly used<br />
in conjunction with Fast Blue. Soluble in: water, lower alcohols<br />
Adv. Cell. Neurobiol., 5, 307 (1984); J. Anat. Soc. India 48(2) 67-72-1999<br />
Catalog # Size<br />
19292-100 100 mg<br />
19292-1 1 g<br />
17316-100 100 mg<br />
17316-1 1 g<br />
09224-10 10 mg<br />
09224-50 50 mg<br />
04001-2 2x25<br />
mg/60 ml<br />
vials<br />
04008-5 5x10 mg<br />
5x30 ml<br />
serum<br />
vials<br />
04001-5 5x25<br />
mg/60 ml<br />
vials<br />
05134-10 10 g<br />
04033-5 5 g<br />
17740-1 1 mg<br />
17740-2 2 mg<br />
17740-5 5 mg
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