I - --ii

Table I
Cultural characteristics of different isolates of A. rabiei.
Isolates' Line Colony Colony Sporula- Size of
Diameter Color tion 2 pycnidia (mm)
(mm)
G-31 G24 55 Light pink ++ 159x 130
G-51 C235 52 - do - ++ 160x 135
G-52 F8 51 - do - ++ 151 x 139
L-24 C235 55 - do - ++ 168 x 143
L-60 Galban 54 - do - ++ 162 x 138
R-21 Pb7 54 - do - ++ 155 x 141
J-101 Pb7 44 Dark brown +++ 240 x 235
G-5 EC 26435 41 - do - +++ 252 x 224
D-30 1-13 42 Mousey grey +++ 163 x 143
L-1 EC 26446 39 Buffy brown ++++ 164 x 149
G-20 V138 40 - do - ++++ 196x 175
G-32 C168 38 Dark brown ++++ 160x 142
I. Isolate codes (the letter stands for location): G=Gurdaspur, L=Ludhiana, J=Jullundur and
D-Delhi.
2. Sporulation ratings: ++ poor, +++ good, ++++ abundant.
G-20 and G-32 which had slow growth, good to abundant sporulation and slightly
bigger pycnidia.
Physiologic Specialization Studies
Chickpea plants of 14 cultivars were grown in 20-cm earthern pots in five
replications. After germination, five plants per pot were allowed to grow for 40
days. The inoculum of A. rabiei was multiplied on chickpea meal agar (chickpea
meal 40 g; agar-agar 20 g; distilled water 1000 ml). Ten-day old cultures of each
isolate were taken from the above media and a spore suspension (10 X 103
spores/mi) was prepared in sterilized distilled water separately for each isolate.
The spore suspension thus prepared for each isolate was sprayed on 40-day old
plants of all 14 cultivars separately. The inoculated plants were kept in chambers
for 48 hours. Disease symptoms started appearing 1 week after inoculation. The
final data regarding stem and foliage infection were recorded 3 weeks after
inoculation. The following 1-9 scale was used for disease rating:
Disease grade Leaf and stem infection
I No infection (highly resistant)
3 Few minute lesions on stem and/or up to 5%foliage infection
(resistant)
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