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indicates that this propagate is more virulent. We still need to use inoculum<br />
from naturally infected tissue (without growing on agar) to know more about<br />
this questions.<br />
M.V. Reddy<br />
None of the varieties identified as resistant so far is free from infection. Stem<br />
lesions with heavy sporulatien causing breakdown is not uncommon. It causes<br />
concern if we believe chickpea lines are genetically homogenous. It is important<br />
to know the reasons for it.<br />
Y.L. Nene<br />
I am interested in your study wherein you showed that isolation from a larger<br />
lesion produced on a resistant line pod developed severe infection on the pods<br />
of the same resistant line. Did you standardize the procedure of inoculation? Is<br />
it possible you used tc ) much inoculum?<br />
S. Hanounik<br />
I used the same level of 1noculum, as I did in my artificial inoculation procedure.<br />
But it is true that (i) our standard artificial procedure introduces new<br />
factors (nutrients etc.), that may influence virulence and (<strong>ii</strong>) when I used the<br />
pathogen from that specific lesion, in this case all my inoculum was from that<br />
virulent type, while our artificial inoculum contained isolates from different<br />
geographical regions.<br />
F.A. Elsayed<br />
The current discussion that reinfection of the same line from special lesions<br />
grown in that line could lead to more or larger lesions than what was originally,<br />
emphasizes the need to identify races and the setting up of differential genotypes<br />
is of greater importance than ever.<br />
S. Hanounik<br />
This is very correct.<br />
Y.L. Nene<br />
I think Dr. Hanounik has raised very interesting points particularly in relation<br />
to the existence of pathogenic variants in fungus population in the same field.<br />
S. Hanounik<br />
I think this is trie because at our Lattakia disease screening site we receive<br />
seeds from different geographical areas and also our artificial inoculun consisted<br />
of isolates from different areas.<br />
J.S. Grewal<br />
How can variation develop in the fungus in the absence of perfect stage of the<br />
fungus?<br />
S. Hanounik<br />
Even in the absence of perfect stage the fungus can develop variation by<br />
muitation, adaptation or other methods at its disposal.
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