Seminar for PhD students - Max-Planck-Institut für biophysikalische ...
Seminar for PhD students - Max-Planck-Institut für biophysikalische ...
Seminar for PhD students - Max-Planck-Institut für biophysikalische ...
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Doktorandenseminar - <strong>Seminar</strong> <strong>for</strong> <strong>PhD</strong> <strong>students</strong><br />
Organizing team: W. Fischle, G. Groenhof, C. Höbartner, S. Jakobs, A. Lange<br />
1). In this genetic screen the Dys and Dg<br />
loss-of-function alleles (Dg 323 , Dg O86 and<br />
DysDf) were used to identify heterozygous<br />
interactions. To find suppressors and/<br />
or enhancers of muscle degeneration we<br />
used both Dg/Dys and RNAi mutants<br />
(Dys RNAi :act-Gal4 and Dg RNAi :tub-Gal4),<br />
which show a moderate degeneration<br />
phenotype (Figure 1, C). The pre-selected<br />
primary screen modifiers were crossed<br />
to Dys and Dg mutants and the muscle<br />
structure of F1 progeny was analyzed. The<br />
paraffin sections of Drosophila indirect<br />
flight muscles were stained with<br />
hematoxyline and eosine and analyzed<br />
utilizing light microscopy. As<br />
a result of the secondary screen, 11<br />
modifiers that enhanced the muscle<br />
degeneration phenotype were identified<br />
(Figure 1, Table 1). Further studies of identified<br />
components showed their requirement<br />
in either muscle or nervous tissue, where<br />
specific interaction with the DGC components<br />
may occur. The novel components<br />
that contribute to DGC dependent muscle<br />
maintenance are being analyzed.<br />
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Fig. 1. Enhancement of muscle degeneration phenotype caused by reduced levels of Dys and Dg. (A)<br />
Location of transverse section of Drosophila indirect flight muscles (IFM). (B) The muscle section of<br />
a wild type fly. (C) The muscle section of Dystrophin RNAi mutant. (D) Enhancement of Dystrophin<br />
phenotype by reduction of one copy of mbl. (E) Enhancement of Dystroglycan phenotype by reduction<br />
of one copy of chif.<br />
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Seite 21<br />
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The Drosophila homologue of the dystrophin<br />
gene - introns containing promoters are major<br />
contributors to the large size of the gene.<br />
FEBS Lett 579: 5365–5371<br />
Shcherbata, HR., Yatsenko, AS., Patterson, L.,<br />
Sood, VD., Nudel, U., Yaffe, D., Baker, D.,<br />
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