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A B der die narkotisierten Tiere oft über Zeiträume von mehreren Stunden präzise und lebenserhaltend gelagert werden müssen. Das mit einem internationalen Patent versehene System wird inzwischen durch die Technologietransfergesellschaft der MPG, die Max-Planck-Innovation GmbH, München betreut. Für dessen bauliche wie konstruktionszeichnerische Umsetzung ebenso wie auch für einige andere der hier gezeigten Arbeiten möchten wir uns bei den Mitarbeitern der Feinmechanischen Werkstätten I (Leiter Rainer Schurkötter), vor allem bei den Herren Andreas Pucher-Diehl, Bernd Henkner und Frank Grabowski sehr bedanken. Unser Dank gilt ebenso Herrn Herbert Nolte (Glastechnik), den Mitarbeitern der Tischlerei (Leiter Peter Böttcher) sowie den Fachkräften der Schlosserei. Seite 10 Abb. 2. (A) Bodenbeheizte Narkosekammer aus rotem Acryl mit Schiebedeckel. Mäuse nehmen die getönte Kammer als dunklen Fluchtort an und laufen zur hinteren Stirnseite, was das Schließen des Schiebedeckels erleichtert. Die Wärmeplatte des Kammerbodens ist einzeln verwendbar (Foto Tammer). (B) Hochwertiger Arbeitstisch mit HPL-Laborarbeitsplatte und integriertem Absaugemodul aus Edelstahl. Die versenkbare Schutzhaube ist zurückgeklappt und kann vollständig unter die Arbeitsplatte versenkt werden (Foto Goldmann). Abb. 3. (A) Die linke Hälfte der Abbildung zeigt ein T2-gewichtetes MR-Bild einer Gehirnhemisphäre der Maus in horizontaler Schichtführung. Die Aufl ösung beträgt 30 µm x 30 µm in der Bildebene und die Schichtdicke 300 µm. In der Bildreihe rechts daneben sind (von oben nach unten) Ausschnittsvergrößerungen der Hirnrinde, des Hippocampus und des Kleinhirns zu sehen (Aufnahme S.Boretius) Rückfragen bitte an: Dr. med. vet. Roland Tammer Biomedizinische NMR Forschungs GmbH Tel.: 1722; E-Mail: rtammer@gwdg.de Originalveröffentlichungen: Boretius S, Kasper L, Tammer R, Michaelis T, Frahm J. MRI of cellular layers in mouse brain in vivo. NeuroImage 2009; doi: 10.1016/j.neuroimage.2009. 05.095. Michaelis T, Boretius S, Frahm J. Localized proton MRS of animal brain in vivo: models of human disorders. J Prog Nucl Magn Reson Spectrosc 2009; 55: 1-34. Es ist alles möglich in diesem Universum. Hauptsache es ist genügend unvernünftig. Niels Bohr
A winning team – magnetic resonance imaging meets precision engineering A tight collaboration between the animal researchers of the Biomedizinische NMR Forschungs GmbH and the mechanical workshop of the MPIbpc resulted in an optimized workfl ow, safety, and animal welfare that led to scientifi c advances in small animal MRI. Scientifi c interest of the biomedical NMR group (head Jens Frahm) focuses on the further development of refi ned magnetic resonance imaging (MRI) methods for noninvasive imaging of inner organs, in particular the brain. While human studies are performed with an MRI system operating at a fi eld strength of 3.0 T, small animal MRI employs both a small-bore system at 2.35 T, which facilitates translational research toward potential clinical applications, and a high-fi eld system at 9.4 T, which optimizes the technical conditions for imaging small structures. A most important issue for studies of the central nervous system of living animals is the development of standardized procedures, including techniques for the immobilization and sustained homeostasis of anesthetized animals. Because commercially available solutions often fail to meet the actual experimental needs, we undertook considerable efforts to design a concept that on the one hand is tailored to our specifi c laboratory conditions, but on the other hand allows for a fl exible adaptation of diverse experimental requirements. All hardware components were developed in close collaboration with the mechanical workshop Feinmechanik I (head Rainer Schurkötter). A key element of our concept was the design and implementation of an MRI-compatible animal and sample carrier system (Fig. 1). It is based on our long-lasting experience in MRI of laboratory animals and fi ts both the low-fi eld and high-fi eld MRI magnets. The system offers a simple exchange of animals and samples, and lends itself to complementary as well as comparative experiments at different fi eld strengths. The patented system is currently managed by Max Planck Innovation, Munich. The mouse carrier system, together with a chamber for anesthesia (Fig. 2) and a device to assist endotracheal intubation (not shown), is part of a “warming chain”. All surfaces in contact with the (premedicated) anesthetized animal are warmed to avoid hypothermia (body temperatures < 36.5 °C). This holds true for preparation periods as well as during MRI examinations, which then may last for several hours. Because animals are anesthetized with volatile anesthetics, the system requires a constant gas fl ow. A specially designed extractor Renovation of the Otto Hahn Library – Last Scene: the Journal Reading Room After more than 12 months of reconstruction work in the administration building and the library, the rebuilding activities are coming close to an end. The last huge action has now been started in the library: the renovation of the journal reading room. For this purpose, all shelves and desks have to be removed including the computers. This means that, unfortunately, they will not be available for the next 3 to 4 weeks. However, all monographs and journals and the copy machines will be accessible. The main entrance of the library is now open again. The back entrance will therefore be shut as of today. In the renovated offi ce opposite to the entrance you fi nd Seite 11 hood (Fig. 3) is part of the workstation and enables endotracheal intubation of the mouse, while avoiding inadequate chronic exposure to waste gas. Not only in industry but also in basic science the organization of an optimized workfl ow and the use of state-of-the-art methodology pave the way to progress. Only recently, our in-house developments together with improved MRI sequences summed up to a fi rst outstanding achievement. Figure 4 shows an in vivo image of the mouse brain with an in-plane resolution of 30 µm x 30 µm, which unravels cell layers in the cortex and subcortical structures (Boretius et al. 2009). It is important to note that realization of a multitude of individual technical solutions within a reasonable time scale could not have been accomplished without the substantial technical skills and support of the Feinmechanik workshop. Special thanks go to Andreas Pucher-Diehl, Bernd Henkner, and Frank Grabowski. We also thank Herbert Nolte (former glas workshop) and the members of the carpentry and machine fi tter’s workshops. our colleagues Renate Haegele, Reinhard Harbaum and Theo Koeppen. Andrea Piepkorn and Bernhard Reuse moved to the new offi ces downstairs in the reading room. Notice will be given immediately after the completion of the renovation work and after the computer workstations will be accessible again. Again we ask you for your patience. Your library team
Seite 1 und 2: Berichte aus den Abteilungen Focusi
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Seite 5 und 6: Christian Wurm studied biology at t
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Seite 9: Erfolgreiche Zusammenarbeit - Metho
Seite 13 und 14: Mehdi Pirouz FG Entwicklungsbiologi
Seite 15 und 16: Publikationen Chinesisches Emeritus
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Seite 19 und 20: Prof. H. Jäckle Seite 19 Dr. H. Os
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