2006 - Institut für Klinische Chemie - UniversitätsSpital Zürich

Universität Zürich > Medizinische Fakultät > Klinische Chemie, Institut für > Prof. Dr.
Arnold von Eckardstein
Lipidomics in clinical diagnostics
Summary / Zusammenfassung
Lipids comprise a family of biomolecules that play prominent roles in many critical metabolic and
biochemical processes such as energy production and storage, the formation and functioning of
cellular membranes, signal transduction, and steroidgenesis. The critical point in studying the invivo
metabolism and the clinical impact of the different lipids is the need of one or several
analytical methods which enable the sensitive, specific determination of the different lipid classes
in their unaltered state. The title which has recently been given to the theme of studying lipids in
their natural environment is lipidomics. As yet only cholesterol and its distribution in different
lipoproteins as well as triglycerides have been used as diagnostic and prognostic markers. We are
interested in the diagnostic and prognostic value of various lipids including oxysterols, bile acids,
phospholipids and sphingolipids in human disease. Liquid chromatography mass spectrometry
(LC-MS/(MS)) methods will be developed and used as analytical tools for the determination of the
various lipid classes in serum and lipoprotein subfractions. Healthy individuals will be analysed to
establish reference values of the different markers and to unravel associations and correlations
with demographic and anthropometric measures as well as clinical biochemical markers. Well
defined patient cohorts will be evaluated to unravel the association of candidate lipids with
diseases, for example cardiovascular or metabolic diseases.
Publications / Publikationen
Burkard I, Rentsch KM, von Eckardstein A (2004) Determination of 24S- and 27hydroxycholesterol
in plasma by high-performance liquid chromatography-mass spectrometry. J
Lipid. Res. 45, 776-781
Burkard I, von Eckardstein A, Rentsch KM (2005) Differentiated quantification fo human bile
acids in serum by high-performance liquid chromatography-tandem mass spectrometry J
Chromatogr. B 826, 147-159
Burkard I, von Eckardstein A, Waeber G, Vollenweider P, Rentsch, KM (2007) Lipoprotein
distribution and biological variation of 24S- and 27-hydroxycholesterol in healthy volunteers.
Atherosclerosis, in press
Keywords / Suchbegriffe
tandem mass spectrometry, liquid chromatography, drug monitoring, metabolomics, lipidomics
Project Leadership and Contacts / Projektleitung und Kontakte
PD Dr. Katharina Rentsch (Project Leader) rentsch@uzh.ch
Prof. Dr. Arnold von Eckardstein (Project Leader) arnold.voneckardstein@ikc.usz.ch
Funding Source(s) / Unterstützt durch
Nachwuchsförderungskredit der Universität Zürich, EU, Foundation
Duration of Project / Projektdauer
Project 3273
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