PROGRAMM JAHRESTAGUNG 2012 30. Nov. – 2. Dez ... - ÖGDV
PROGRAMM JAHRESTAGUNG 2012 30. Nov. – 2. Dez ... - ÖGDV
PROGRAMM JAHRESTAGUNG 2012 30. Nov. – 2. Dez ... - ÖGDV
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Poster Wundheilung<br />
P 50<br />
Secretome of peripheral blood mononuclear cells enhances wound healing<br />
Michael Mildner 1<br />
Stefan Hacker 2<br />
Thomas Haider 3<br />
Caterina Barresi 1<br />
Matthias Zimmermann 3<br />
Bahar Golabi 3<br />
Erwin Tschachler 1<br />
Hendrik Ankersmit 3<br />
1 Department of Dermatology, Medical University Vienna, Austria<br />
2 Department of Plastic Surgery, Medical University Vienna, Austria<br />
3 Department of Thoracic Surgery, Medical University Vienna, Austria<br />
Introduction: Non-healing skin ulcers are often resistant to most common treatments.<br />
Treatment with growth factors has been proven to improve closure of chronic wounds.<br />
Here we investigate whether lyophilized culture supernatant of freshly isolated<br />
peripheral blood mononuclear cells (PBMCs) is able to enhance wound healing.<br />
Methods: PBMCs from healthy human individuals were prepared and cultured for 24<br />
hours. Supernatants were collected, dialyzed and lyophilized (Livosec). Six mm punch<br />
biopsy wounds were set on the backs of C57BL/6J-mice. Morphology and neoangiogenesis<br />
were analyzed by H&E-staining and CD31 immuno-staining, respectively.<br />
In vitro effects on diverse skin cells were investigated by migration assays, cell cycle<br />
analysis and tube formation assay. Signaling pathways were analyzed by Western blot<br />
analysis.<br />
Results: Application of Livosec on 6 mm punch biopsy wounds significantly enhanced<br />
wound-closure. H&E staining of the wounds after 6 days revealed that wound healing<br />
was more advanced after application of Livosec containing emulsion. Furthermore,<br />
there was a massive increase in CD31 positive cells, indicating enhanced neoangiogenesis.<br />
In primary human fibroblasts (FB) and keratinocytes (KC) migration but<br />
not proliferation was induced. In endothelial cells (EC) Livosec induced proliferation<br />
and tube-formation in a matrigel-assay. In addition, Livosec treatment of skin cells led<br />
to the induction of multiple signaling-pathways involved in cell migration, proliferation<br />
and survival. In summary, these results suggest that emulsions containing Livosec<br />
accelerate wound healing in a mouse model and induce wound healing associated<br />
mechanisms in human primary skin cells. The formulation and use of such emulsions<br />
might therefore represent a possible novel option for the treatment of non-healing skin<br />
ulcers.<br />
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