Angelika Semmler - KOPS - Universität Konstanz

8 Experimenteller Teil
Cyclo[Pll-Gly-Pro-Gly-Ala-Phe-Glu]-Lys-NH2 (19→8)
Analytische RP-HPLC (Nucleosil Säule): (10-85% B in 30 min, 1ml/min)
RP-HPLC / tR ESI-MS gemessen [M+X] + berechnet
22,4 min 1246,8 m/z [M+H] +
Peptid 9
Fmoc-Gly-Pro-Ala-Gly-Phe-Glu(OAll)-Lys(Boc)-NH2
1246,57 m/z (C63H80N11O16 + )
Analytische RP-HPLC (Nucleosil Säule): (10-85% B in 30 min, 1 ml/min)
RP-HPLC / tR MALDI-TOF-MS gemessen [M+X] + berechnet
24,6 min 1089,1 m/z [M+Na] + 1088,50 m/z (C55H71N9NaO13 + )
20,4 min 966,7 m/z [(M-Boc)+H] + 966,47 m/z (C50H64N9O11 + )
Fmoc-Pll-Gly-Pro-Ala-Gly-Phe-Glu(OAll)-Lys-NH2
Analytische RP-HPLC (Nucleosil Säule): (10-85% B in 30 min, 1 ml/min)
RP-HPLC / tR MALDI-TOF-MS gemessen [M+X] + berechnet
22,4 min 1247,7 m/z [M+H] + 1246,57 m/z (C63H80N11O16 + )
Cyclo[Pll-Gly-Pro-Ala-Gly-Phe-Glu]-Lys(Boc)-NH2 (9)
Analytische RP-HPLC (Nucleosil Säule): (10-85% B in 30 min, 1 ml/min)
RP-HPLC / tR ESI-MS gemessen [M+X] + berechnet
13,7-14,2 min 966,4 m/z [(M-Boc)+H] + 966,46 m/z (C45H71N11O13 + )
Peptid 10
Fmoc-Pll-Gly-Ala-Pro-Gly-Phe-Glu(OAll)-Lys-NH2
Analytische RP-HPLC (Nucleosil Säule): (10-85% B in 30 min, 1 ml/min)
RP-HPLC / tR MALDI-TOF-MS gemessen [M+X] + berechnet
25,5 min 1247,2 m/z [M+H] + 1246,57m/z (C63H80N11O16 + )
Cyclo[Pll-Gly-Ala-Pro-Gly-Phe-Glu]-Lys(Boc)-NH2 (10)
Analytische RP-HPLC (Nucleosil Säule): (10-85% B in 30 min, 1 ml/min)
RP-HPLC / tR ESI-MS gemessen [M+X] + berechnet
18,2-18,3 min 1066,4 m/z [M+H] + 1066,52 m/z (C50H72N11O15 + )
14,1-14,7 min 966,4 m/z [(M-Boc)+H] + 966,46 m/z (C45H71N11O13 + )
132