Arbeitsanleitung - Milenia Biotec GmbH
Arbeitsanleitung - Milenia Biotec GmbH
Arbeitsanleitung - Milenia Biotec GmbH
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Summary and Explanation<br />
Being an acute phase protein, C-reaktive protein (CRP) is mainly synthesized in the liver. However, recent<br />
research has shown that the inflammatory protein is also produced in other tissues such as vascular smooth<br />
muscle cells and adipocytes. The expression of CRP is particularly increased in pathologically altered vessels.<br />
The CRP serum level was originally determined to detect and monitor acute inflammation. The value may<br />
increase up to 1000fold under acute inflammatory conditions.<br />
Method and Test Principle<br />
The <strong>Milenia</strong> ® QuickLine CRP test is a lateral flow immunoassay designed for semi quantitative measurement<br />
of human CRP in capillary blood, venous blood, or serum.<br />
The sample is pipetted into the sample application port.<br />
The CRP in patients’ sample binds first to a monoclonal anti-CRP antibody that is conjugated to gold particles.<br />
The CRP-loaded gold particles diffuse through the membrane and pass Test line T. There, a monoclonal CRP<br />
specific antibody is coated on the membrane. At this place the CRP-gold-particels were bound specifically and<br />
become visible as Test line T. The color intensity of the Test line is directly proportional to the concentration of<br />
CRP in the sample. The intensity increases during incubation time. Excess gold conjugate diffuses further over<br />
the test membran. Conjugat-specific antibodies that applied onto the membrane are functioning as Control line<br />
(C). Here the residual gold conjugate is captured and forms a well visible line during the incubation time.<br />
Increasing or decreasing colour’s intensity of the Control line can not be used for any interpretation.<br />
The test unit must be evaluated colourimetrically with the <strong>Milenia</strong> ® POCScan Reader.<br />
The result displayed on the reader is calculated according to a stored standard curve.<br />
<strong>Milenia</strong> <strong>Biotec</strong>, Versailler Straße 1, D-35394 Gießen<br />
<strong>Milenia</strong> QuickLine CRP (MQLRP)