Arbeitsanleitung - Milenia Biotec GmbH

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Summary and Explanation
Being an acute phase protein, C-reaktive protein (CRP) is mainly synthesized in the liver. However, recent
research has shown that the inflammatory protein is also produced in other tissues such as vascular smooth
muscle cells and adipocytes. The expression of CRP is particularly increased in pathologically altered vessels.
The CRP serum level was originally determined to detect and monitor acute inflammation. The value may
increase up to 1000fold under acute inflammatory conditions.
Method and Test Principle
The Milenia ® QuickLine CRP test is a lateral flow immunoassay designed for semi quantitative measurement
of human CRP in capillary blood, venous blood, or serum.
The sample is pipetted into the sample application port.
The CRP in patients’ sample binds first to a monoclonal anti-CRP antibody that is conjugated to gold particles.
The CRP-loaded gold particles diffuse through the membrane and pass Test line T. There, a monoclonal CRP
specific antibody is coated on the membrane. At this place the CRP-gold-particels were bound specifically and
become visible as Test line T. The color intensity of the Test line is directly proportional to the concentration of
CRP in the sample. The intensity increases during incubation time. Excess gold conjugate diffuses further over
the test membran. Conjugat-specific antibodies that applied onto the membrane are functioning as Control line
(C). Here the residual gold conjugate is captured and forms a well visible line during the incubation time.
Increasing or decreasing colour’s intensity of the Control line can not be used for any interpretation.
The test unit must be evaluated colourimetrically with the Milenia ® POCScan Reader.
The result displayed on the reader is calculated according to a stored standard curve.
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Milenia QuickLine CRP (MQLRP)