HPLC-Analytik Adenosin - Labodia
HPLC-Analytik Adenosin - Labodia
HPLC-Analytik Adenosin - Labodia
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<strong>HPLC</strong>-<strong>Analytik</strong> <strong>Adenosin</strong><br />
Chromatographic conditions<br />
Column material: Nucleosil 100 C18, 10 µm<br />
Column dimension: 125 x 4 mm<br />
Flow rate: 1.0 ml/min<br />
UV-Detection: 254 nm<br />
Temperature: 30 °C<br />
Injection volume: 100 µl<br />
Running time/sample: 35 min<br />
Gradient: 0 min (100 % A / 0 % B)<br />
12 min (100 % A / 0 % B)<br />
24 min (60 % A / 40 % B)<br />
26 min (0 % A / 100 % B)<br />
28 min (0 % A / 100 % B)<br />
29 min (100 % A / 0 % B)<br />
35 min (100 % A / 0 % B)<br />
We recommend strongly the use of a pre-column to prolong the life of<br />
the analytical column.<br />
10. TREATMENT OF THE COLUMN<br />
After each run, the column should be washed with ca. 50 ml of aqua bidist.<br />
at a flow rate of 1 ml/min. Afterwards, the column is stored in 85%<br />
Acetonitril / 15% aqua bidist. (ca. 30 ml, flow rate 1.0 ml/min).<br />
Before use, the system should be equilibrated with ca. 30 ml mobile phase A<br />
(MOPHA).<br />
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