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Herbsttagung Programm & Abstracts - Deutsche Gesellschaft für ...

Herbsttagung Programm & Abstracts - Deutsche Gesellschaft für ...

Herbsttagung Programm & Abstracts - Deutsche Gesellschaft für ...

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A novel human short term ex vivo model for the initial phase of mycobacterial<br />

infection<br />

Ganbat D 1 , Richter E 1 , Vollmer E 1 , Zabel P 1 , Kugler C 2 , Goldmann T 1<br />

1 Research Center Borstel, D-23845 Borstel; 2 LungenClinic Grosshansdorf, D-22927 Großhansdorf<br />

Introduction: We present the development of a novel human ex vivo tissue culture model for the<br />

simulation of the initial phase of mycobacterial infections. This model is designated STST (Short Term<br />

Stimulation of Tissues) and is intended to be used for the determination of the host-reaction upon<br />

mycobacterial infection and was previously described for Haemophilus influenza, Chlamydia<br />

pneumonia and Streptococcus pneumoniae.<br />

Methods: After ex vivo infection, the tissues are preserved and paraffin-embedded by applying the<br />

HOPE-technique. Different conditions are checked to obtain optimal results in terms of bacterial dose,<br />

incubation time etc.. In this model we plan to apply transcriptome analysis in order to comprehensively<br />

study the reaction of the host. After that we will validate the results in a large scale series under<br />

inclusion of samples from TB-patients.<br />

Results: The results obtained in the course of establishing the model show nicely preserved<br />

morphology and a clear localization of mycobacteria intracellular within the tissues.<br />

Discussion: The ex vivo model will serve to gain new insights into the complex interactions taking<br />

place in the human lung upon infection with mycobacteria.<br />

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