Herbsttagung Programm & Abstracts - Deutsche Gesellschaft für ...
Herbsttagung Programm & Abstracts - Deutsche Gesellschaft für ...
Herbsttagung Programm & Abstracts - Deutsche Gesellschaft für ...
Sie wollen auch ein ePaper? Erhöhen Sie die Reichweite Ihrer Titel.
YUMPU macht aus Druck-PDFs automatisch weboptimierte ePaper, die Google liebt.
A novel human short term ex vivo model for the initial phase of mycobacterial<br />
infection<br />
Ganbat D 1 , Richter E 1 , Vollmer E 1 , Zabel P 1 , Kugler C 2 , Goldmann T 1<br />
1 Research Center Borstel, D-23845 Borstel; 2 LungenClinic Grosshansdorf, D-22927 Großhansdorf<br />
Introduction: We present the development of a novel human ex vivo tissue culture model for the<br />
simulation of the initial phase of mycobacterial infections. This model is designated STST (Short Term<br />
Stimulation of Tissues) and is intended to be used for the determination of the host-reaction upon<br />
mycobacterial infection and was previously described for Haemophilus influenza, Chlamydia<br />
pneumonia and Streptococcus pneumoniae.<br />
Methods: After ex vivo infection, the tissues are preserved and paraffin-embedded by applying the<br />
HOPE-technique. Different conditions are checked to obtain optimal results in terms of bacterial dose,<br />
incubation time etc.. In this model we plan to apply transcriptome analysis in order to comprehensively<br />
study the reaction of the host. After that we will validate the results in a large scale series under<br />
inclusion of samples from TB-patients.<br />
Results: The results obtained in the course of establishing the model show nicely preserved<br />
morphology and a clear localization of mycobacteria intracellular within the tissues.<br />
Discussion: The ex vivo model will serve to gain new insights into the complex interactions taking<br />
place in the human lung upon infection with mycobacteria.<br />
20