Tierärztliche Hochschule Hannover - TiHo Bibliothek elib

Tab. 2: Suitability of various techniques to reduce autofluorescence in
formalin-fixed pancreatic tissue sections
To compare the ability to quench autofluorescence, we applied the Sudan Black B
protocol in parallel to other methods as indicated. Using different fluorescence filter
setups, the remaining mean autofluorescence level was evaluated by quantitative
analysis of identically sized image areas that display comparable exocrine tissue
areas rich in acini. For each experiment, mean autofluorescence of control images
obtained from specimen without treatment was defined as 100% intensity. The
corresponding experiments are described in Fig. 6, 7, and 8.
Detected tissue autofluorescence
Tissue treatment DAPI filter FITC filter Cy3 filter
Sudan Black compared to Toluidine Blue approach
no treatment 100.0 % 100.0 % 100.0 %
Toluidine Blue treatment 128.8 % 32.1 % 147.3 %
Sudan Black +
Toluidine Blue
38.2 % 21.6 % 166.5 %
Sudan Black treatment 32.0 % 5.1 % 21.6 %
Sudan Black compared to cupric sulphate approach
no treatment 100.0 % 100.0 % 100.0 %
100 mM cupric sulphate 54.9 % 30.1 % 40.5 %
Sudan Black treatment 34.3 % 14.2 % 19.0 %
Sudan Black compared to photobleaching approach
no treatment 100.0 % 100.0 % 100.0 %
UV-irradiation 85.6 % 60.5 % 58.3 %
Sudan Black treatment 27.4 % 23.5 % 21.0 %
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