Tierärztliche Hochschule Hannover - TiHo Bibliothek elib

For each set of compared experiments the images were acquired with identical
microscopic equipment and settings and selected image areas were identical in size.
The mean fluorescence emisson intensity of untreated tissue specimen was defined
as a 100% reference value and fluorescence intensities of sections after treatments
were rated proportionally.
Percentage of autofluorescence of distinct tissue and cellular structures, such as
blood vessels, loose or tight connective tissue, erythrocytes or zymogen granuleenriched
areas, was performed by first defining a narrow region of interest (e.g. Fig.
1, Fig. 3; marked area between white lines) and calculating the mean intensity profile
along this region (Cell^F imaging software). In untreated tissue sections the
fluorescence intensity for the position of a histological structures of interest (as shown
in Fig. 1 E; Fig. 3 I-J) was defined as reference value of 100%. Adjacent slices of
serial sections were treated to quench autofluorescence and the intensity profile of
the respective position displaying identical tissue constituents was rated
proportionally.
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