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H 20 REFERENCE GUIDE/ALLGEMEINE INFORMATION INSTRUMENT CLEANING AND STERILIZATION Instrument Cleaning All instruments need to be cleaned and thoroughly dried before they are sterilized. They should be washed with a non-corrosive, low sudsing neutral detergent. Instrument cleaning can be accomplished by ultrasonic or automated cleaning, which is preferred to minimize the opportunity of sharps injuries due to hand scrubbing. Hu-Friedy does not suggest the use of any abrasive brushes or materials to clean instruments. Instrument Sterilization Sterilization is a process that kills microorganisms. There are three common methods of heat sterilization used in the dental office that can be verified by spore testing (steam autoclave and chemical vapor). The following chart outlines sterilization methods: Comparison of Heat Sterilization Methods Comparison of Cleaning Methods Method Advantages Disadvantages Hand scrubbing • Effective if performed • Increases chances for operator injury properly • Increases spread of contamination • through splatter • Labor-intensive • Need proper care of scrub brush Ultrasonic cleaning • Safer than hand scrubbing • Microorganisms may accumulate in • Effectively cleans all cleaning solution instruments • Reduces chances for spread of contaminants through splatter • Allows for more efficient use of staff time • Ultrasonic cleaning will not removehardened permanent cement. (Solution: remove cement while it is still soft.) Automated washer • Safer than hand scrubbing • Not all instruments are compatible • Reduces chances for spread with automated washers. Please see of contaminants through manufacturer’s instructions for splatter and aerosols detailed requirements. • Allows for more efficient use of staff time • Effectively cleans instruments Method Standard Sterilization Advantages Precautions Spore Testing Conditions* Steam autoclave 20 minutes at 121˚C • Time efficient • Do not use closed containers Bacillus stearothermophilus + 5 minutes at 132/134°C • Good penetration • May damage plastic and rubber strips, vials, or ampules (15 psi) • Sterilize water-based liquid items • Non-stainless steel metal items corrode • Use of hard water may leave deposits • Dry instruments Unsaturated 20 minutes at 132˚C • Time efficient • Do not use closed containers Bacillus stearothermophilus chemical vapor (20–40 psi) • No corrosion • May damage plastic and rubber strips • Items dry quickly after cycle items • Must use special solution • Dry instruments or dip in special solution • Provide adequate ventilation • Cannot sterilize liquids *These conditions do not include warm-up time and they may vary depending on the nature and volume of the load. Sterilizing conditions in your office should be defined by results of routine spore-testing. Check guidelines from your specific manufacturer. Adapted from Miller CH: Take the safe approach to prevent disease transmission. RDH 95:35, 1989; with permission.

REINIGEN UND STERILISIEREN ZAHNÄRZTLICHER INSTRUMENTE Reinigen zahnärztlicher Instrumente Vor dem Sterilisieren müssen die Instrumente gereinigt werden. Dazu sind die Instrumente mit einem nicht aggressiven, schwach schäumenden neutralen Reinigungsmittel abzuwaschen. Die Instrumente können von Hand, im Ultraschallbad oder im Thermodesinfektor gereinigt werden. Sterilisieren zahnärztlicher Instrumente Beim Sterilisieren werden sämtliche Mikroorganismen abgetötet. Im allgemeinen kommen für den Praxisbetrieb drei Sterilisationsverfahren in Betracht, deren Wirksamkeit durch Sporenprobe überprüft werden kann: Autoclav - Chemieklav. In folgender Tabelle werden diese Verfahren beschrieben. Verfahren zur Hitzesterilisierung Reinigungsverfahren Verfahren Vorteile Nachteile Reinigen von Hand • bei sorgfältiger Ausführung • erhöhtes Verletzungsrisiko effektive Wirkung beim Reinigen • Kontaminationsrisiko durch verschüttetes/spritzendes Reinigungsmittel • arbeitsaufwendig • Bürste muß sachgemäß gepflegt werden. Ultraschall-Reinigung • sicherer als Reinigen • Mikroorganismen können sich in von Hand Reinigungslösung ansammeln. • hoher Wirkungsgrad • reduziertes Kontaminationsrisiko • Zeitersparnis Thermodesinfektor • sicherer als Reinigen • größere Menge muß angesammelt von Hand werden, um Reinigungsprozeß • reduziertes zu starten. Kontaminationsrisiko • Zeitersparnis Verfahren Standard-Sterilisie- Vorteile Vorsichtsmaßnahmen Sporenprobe rungs-Bedingungen* Autoclav 20 Minuten bei 121°C; • Zeitersparnis • keine geschlossenen Bacillus stearothermophilus + 5 Minuten bei 132/134°C • gute Tiefenwirkung Behälter sterilisieren (Streifen, Phiolen (15 psi) • Sterilisation mit Wasserdampf • Kunststoff- und Gummiteile oder Ampullen) können beschädigt werden • Metall-Teile können korrodieren • hartes Wasser kann Kalkablagerungen zur Folge haben Chemieklav 20 Minuten bei 132°C; • Zeitersparnis • keine geschlossenen Bacillus stearothermophilus (20-40 psi) • keine Korrosion Behälter sterilisieren (Streifen) • Instrumente trocknen rasch • Kunststoff- und Gummiteile können beschädigt werden • Speziallösung erforderlich • Instrumente vortrocknen oder in Speziallösung tauchen • angemessene Belüftung sicherstellen • keine Flüssigkeiten sterilisieren *Ohne Aufheizzeit; die Zyklusdauer kann je nach Art und Volumen des zu sterilisierenden Materials schwanken. Die Sterilisationsbedingungen sollten individuell aufgrund regelmäßiger Sporenproben bestimmt werden. Mit freundlicher Genehmigung nach Miller, CH.: Take the safe approach to prevent disease transmission., RDH 95:35, 1989. H 21

H 20 REFERENCE GUIDE/ALLGEMEINE INFORMATION<br />

INSTRUMENT CLEANING AND STERILIZATION<br />

Instrument Cleaning<br />

All instruments need to be cleaned<br />

and thoroughly dried before they are<br />

sterilized. They should be washed<br />

with a non-corrosive, low sudsing<br />

neutral detergent. Instrument cleaning<br />

can be accomplished by ultrasonic or<br />

automated cleaning, which is preferred<br />

to minimize the opportunity of sharps<br />

injuries due to hand scrubbing.<br />

Hu-Friedy does not suggest the use<br />

of any abrasive brushes or materials to<br />

clean instruments.<br />

Instrument Sterilization<br />

Sterilization is a process that kills<br />

microorganisms. There are three common<br />

methods of heat sterilization used in the<br />

dental office that can be verified by<br />

spore testing (steam autoclave and<br />

chemical vapor). The following chart<br />

outlines sterilization methods:<br />

Comparison of Heat Sterilization Methods<br />

Comparison of Cleaning Methods<br />

Method Advantages Disadvantages<br />

Hand scrubbing • Effective if performed • Increases chances for operator injury<br />

properly • Increases spread of contamination<br />

• through splatter<br />

• Labor-intensive<br />

• Need proper care of scrub brush<br />

Ultrasonic cleaning • Safer than hand scrubbing • Microorganisms may accumulate in<br />

• Effectively cleans all cleaning solution<br />

instruments<br />

• Reduces chances for<br />

spread of contaminants<br />

through splatter<br />

• Allows for more efficient<br />

use of staff time<br />

• Ultrasonic cleaning will not<br />

removehardened permanent<br />

cement. (Solution: remove cement<br />

while it is still soft.)<br />

Automated washer • Safer than hand scrubbing • Not all instruments are compatible<br />

• Reduces chances for spread with automated washers. Please see<br />

of contaminants through manufacturer’s instructions for<br />

splatter and aerosols detailed requirements.<br />

• Allows for more efficient use<br />

of staff time<br />

• Effectively cleans instruments<br />

Method Standard Sterilization Advantages Precautions Spore Testing<br />

Conditions*<br />

Steam autoclave 20 minutes at 121˚C • Time efficient • Do not use closed containers Bacillus stearothermophilus<br />

+ 5 minutes at 132/134°C • Good penetration • May damage plastic and rubber strips, vials, or ampules<br />

(15 psi) • Sterilize water-based liquid items<br />

• Non-stainless steel metal items<br />

corrode<br />

• Use of hard water may leave<br />

deposits<br />

• Dry instruments<br />

Unsaturated 20 minutes at 132˚C • Time efficient • Do not use closed containers Bacillus stearothermophilus<br />

chemical vapor (20–40 psi) • No corrosion • May damage plastic and rubber strips<br />

• Items dry quickly after cycle items<br />

• Must use special solution<br />

• Dry instruments or dip in<br />

special solution<br />

• Provide adequate ventilation<br />

• Cannot sterilize liquids<br />

*These conditions do not include warm-up time and they may vary depending on the nature and volume of the load. Sterilizing conditions in<br />

your office should be defined by results of routine spore-testing. Check guidelines from your specific manufacturer.<br />

Adapted from Miller CH: Take the safe approach to prevent disease transmission. RDH 95:35, 1989; with permission.

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