Protocol for RiboShredder™ RNase Blend

EPICENTRERiboShredder RNase BlendRiboShredder Protocol(removal of RNA from DNA Preparations)1. Extract the DNA from a 1-2 ml overnight bacterial culture using the nucleic acid purification methodof your choice.2. After ethanol precipitation, resuspend the final nucleic acid pellet in T 10 E 1 Buffer (10 mM Tris-HCl[pH 7.5, 25 o C], 1 mM EDTA) at a concentration appropriate for subsequent applications.3. Add 1 ml of RiboShredder RNase Blend per 130 µg total nucleic acids to each reaction.4. Incubate the reaction at 37 o C for 10 minutes.5. Remove RiboShredder RNase Blend by phenol/chloroform extraction. Precipitate the DNA usingan ethanol precipitation.NOTE: Reactions may be scaled up as needed. Use 1 U of RiboShredder RNase Blend/130 mg totalnucleic acids in the sample.page 2 10/05